Lowe, journallab on a chip, year20, volume 12, pages 221051. A accommodates more assays on the same footprint of a device than a 2d device testing of different samples simultaneously within a compact space b moves fluid through the thickness of paper the zdirection and laterally the x,yplane. A chemically patterned microfluidic paperbased analytical. There was a dramatic rise in the use of paper in point of care diagnostics after two reports in 2007 demonstrated that. A paperbased multiplexed transaminase test for lowcost. In fact, they can not only be used for biochemical analysis but also for medical and forensic diagnostics. Pdf a historical perspective on paper microfluidic based. Paper based microfluidic point of care diagnostic devices received 6th february 20, accepted 18th march 20 doi. Paperbased microfluidics are microfluidic devices that consist of a series of hydrophilic cellulose or nitrocellulose fibers that guide liquid from an inlet to a desired outlet by imbibition. Pointofcare poc diagnostics play an important role in delivering healthcare, particularly for clinical management and disease surveillance in both developed and developing countries. Paper based microfluidic systems have emerged as one of the most favorable technologies used in many potential applications such as point of care diagnostics, flexible electronics, energy storage. The overall goal of this dissertation is to develop microfluidic diagnostic devices which are practical and reliable for global health. Paperbased analytical devices for clinical diagnosis.
However, paperbased microfluidics can enable fluid handling and quantitative analysis for potential applications in healthcare, veterinary medicine, environmental. Early microfluidicbased pointofcare diagnostic devices included glucose monitors, which have become a standard commodity because they are inexpensive to make and they work well, according to yager. Paper substrates have been widely used to construct point of care lateral flow immunoassay diagnostic devices like the home pregnancy test. Special issue paperbased microfluidic devices for point. In 2008, the group introduced the first 3d microfluidic paperbased analytical devicesalso referred to as pads. Cheap ubiquitous lightweight can be disposed of by incineration, so doesnt produce biohazardous waste. Pointofcare microfluidic devices for pathogen detection. Recently, flexible polymer substrates have been investigated due to. Mar 22, 2017 numerous health care applications have stemmed from supplementing external attachments to smartphones 2325, with multiple pointofcare diagnostic devices using the onboard smartphone camera 2527. Microfluidic and paperbased devices for disease detection. Recently, paper has been functionalised as a substrate to construct microfluidic devices for use in rapid diagnostic tests. The global microfluidics market size was evaluated at usd. Portable, lowcost, and quantitative nucleic acid detection is desirable for pointofcare diagnostics. Microfluidic paperbased analytical devices pads have provided a breakthrough in portable and lowcost point of care diagnostics.
Paperbased analytical devices pads as demonstrated by whitesidess group offer a promising solution for pointofcare diagnostics 2527. Progress in the development of paperbased diagnostics for. To tune fluid penetration in porous substrates such as paper in two and three dimensions, the pore structure, wettability and geometry of the microfluidic devices can be controlled. Pads are expected to have profound implications, especially for in vitro diagnosis. Whitesides5 in developed nations, monitoring for dr uginduced liver injury through serial. Fabrication of miniaturized paperbased microfluidic. Aug 22, 2017 this work describes the fabrication of self. The goal was to develop lowcost and portable paperbased diagnostic devices to. In addition, the basics of paperbased microfluidics including a short history of paperbased microfluidics, a comparison of paper based and conventional plastic or glass based microfluidic devices and general requirements for ideal point of care testing devices are described. Salivary diagnostics on paper microfluidic devices and. Paper based microfluidics are microfluidic devices that consist of a series of hydrophilic cellulose or nitrocellulose fibers that guide liquid from an inlet to a desired outlet by imbibition.
Nevertheless, only singleanalyte approaches are typically. The main advantage of this is that it is largely a passively. Paperbased devices are a portable, userfriendly, and affordable technology that is one of the best analytical tools for inexpensive diagnostic devices. The first system, using digital microfluidics, is reusable and allows for precise fluid control. Pads can provide potentially relevant analysis for biomarker detection in many types of biological fluids, including tear fluids 28, 29, whole blood 30 32, serum 33, urine 34 and saliva. Apr 26, 2019 in addition, the basics of paper based microfluidics including a short history of paper based microfluidics, a comparison of paper based and conventional plastic or glass based microfluidic devices and general requirements for ideal point of care testing devices are described. Beattie,2 sidhartha jain,2 farzad noubary,3 vicki l. The integration issues relevant to the creation of paperbased diagnostic devices are analogous to the issues in conventional microfluidicsbased diagnostic device development. Pads and wearable devices have been highly studied to be used as diagnostic tools due to their advantages such as simplicity and ability to provide instrumentfree fast results. Pdf paperbased microfluidic pointofcare diagnostic. There was a dramatic rise in the use of paper in pointofcare diagnostics after two reports in 2007 demonstrated that. We report an integrated microfluidic diagnostic device capable of onsite quantitative nucleic acid detection directly from the blood without separate sample preparation steps.
Paperbased microfluidic devices are robust and relatively simple to operate, compared to channel microfluidic devices, which is perhaps their greatest advantage and the reason they have reached a high. Successful device integration can be achieved by a twopart approach composed of a systemlevel view of the whole device and individual module development 84. Speds are inexpensive, lightweight, mechanically flexible, easy to use, and disposable by burning. Apr 26, 2017 a chemically patterned microfluidic paperbased analytical device cpad is developed to create fluidic networks by forming hydrophobic barriers using chemical vapor deposition cvd of.
The expanding role of paper in pointofcare diagnostics. Development of paper based point of care microfluidic diagnostic devices project number imura0628 monash main supervisor name, email id, phoneadrian neild, adrian. Paper based microfluidic devices fill a growing niche for portable, cheap, and userfriendly medical diagnostic systems. Fabrication of laser printed microfluidic paperbased. Point of care poc diagnostics play an important role in delivering healthcare, particularly for clinical management and disease surveillance in both developed and developing countries. Paper based microfluidic devices are robust and relatively simple to operate, compared to channel microfluidic devices, which is perhaps their greatest advantage and the reason they have reached a high. Development of pointofcare diagnostic assays using paperbased microfluidic devices professor charles mace tufts university, department of chemistry abstract the ability to perform diagnostic assays simply and expediently at the pointofcare has broad implications for the global management of healthcare. This platform opens new possible applications for paper based point of care poc diagnostic devices and enables the possibility of these devices to introduce time control functions without the.
An automated smartphonebased diagnostic assay for point. Over the last decade, one particularly promising realworld application to emerge has been point of care poc diagnostics. Microfluidicbased pointofcare testing for global health tassaneewan laksanasopin. There is a wide variety of papers that can be employed to build microfluidic devices. But his teams aim is to enable microfluidics to perform a greatly expanded range of tests for everything from infectious diseases to drug. Can be produced on a mass scale portable 6 what are microfluidic pointofcare diagnostic devices in the year 2004, the world. However, paper based microfluidics can enable fluid handling and quantitative analysis for potential applications in healthcare, veterinary medicine, environmental. Diagnostics for all, instrumentfree tests based on paper, capillarydriven microfluidics, with a colorimetric readout. Recently, paperbased microfluidics has emerged as a multiplexable pointofcare platform which might transcend the capabilities of existing assays in resourcelimited settings. Rise in demand for point of care poc devices is expected to significantly drive the market. The goal was to develop lowcost and portable paperbased diagnostic devices to improve healthcare in developing countries. The future of microfluidic pointofcare diagnostic devices.
Jun 28, 2010 early microfluidic based point of care diagnostic devices included glucose monitors, which have become a standard commodity because they are inexpensive to make and they work well, according to yager. In addition, the basics of paperbased microfluidics including a short history of paperbased microfluidics, a comparison of paperbased and conventional plastic or glassbased microfluidic devices and general requirements for ideal pointofcare testing devices are described. Numerous health care applications have stemmed from supplementing external attachments to smartphones 2325, with multiple pointofcare diagnostic devices using the onboard smartphone camera 2527. We define paper broadly as a variety of porous materials, although the emphasis often is on traditional cellulosic forms.
Paper based microfluidics rely on the phenomenon of capillary penetration in porous media. Paperbased microfluidic devices have a great potential to be a good alternative to traditional microfluidic systems for pointofcare diagnostic applications. Apr 12, 2016 3d paper based microfluidics have the following advantages over 2d. Apr 26, 2017 multiplexed pointofcare testing xpoct, which is simultaneous onsite detection of different analytes from a single specimen, has recently gained increasing importance for clinical diagnostics, with emerging applications in resourcelimited settings such as in the developing world, in doctors offices, or directly at home.
Paperbased microfluidic pointofcare diagnostic devices. Multiplexed pointofcare testing xpoct, which is simultaneous onsite detection of different analytes from a single specimen, has recently gained increasing importance for clinical diagnostics, with emerging applications in resourcelimited settings such as in the developing world, in doctors offices, or directly at home. Since their introduction in 2007, paperbased microfluidic devices micropads have been explored extensively as platforms for pointofcare diagnostic tests and as tools for basic research and. Microfluidicbased pointofcare testing for global health. Despite their significant scope, the complexity of fabrication. Odevelopment of pointofcare diagnostic assays using. Biological detection biological activities can be recognized by different signal transduction pathways performing on the. A microfluidic paper based analytical device pad presents a promising alternative to traditional laboratory tests by allowing for the rapid and lowcost diagnoses of diseases in resource. Making paper diagnostic tests, made while he was working.
While many devices focus on one of those four areas of detection, a few devices identifies fungi such as candida. Moreover, each device can be used to measure more than one analyte. A large part of the excitement behind microfluidics is in its potential for producing practical devices, but surprisingly few labonachip based technologies have been successfully introduced into the market. As a rapidly developing field, paperbased microfluidic devices. Diseases such as periodontitis and diabetes mellitus can potentially be detected through these devices by the detection of important biomarkers. Selfpowered integrated microfluidic pointofcare low. Recently, paper based microfluidics has emerged as a multiplexable point of care platform which might transcend the capabilities of existing assays in resourcelimited settings. Pads are an evolution of single layer devices and they permit effective sample dispersion, individual layer treatment, and multiplex analytical assays. Development of point of care diagnostic assays using paper based microfluidic devices professor charles mace tufts university, department of chemistry abstract the ability to perform diagnostic assays simply and expediently at the point of care has broad implications for the global management of healthcare. Paperbased pointofcare testing in disease diagnostics. Digital and paper microfluidic diagnostic devices design document 3 summary the project aims to design two complementary systems for performing biological assays and diagnostic tests. Pads are a new class of pointofcare diagnostic devices that are inexpensive.
Paperbased point of care diagnostics posner research group. Biomedical engineering a paperbased multiplexed transaminase test for lowcost, pointofcare liver function testing nira r. The technology builds on the conventional lateral flow test which is capable of detecting many infections agents and chemical contaminants. Paperbased 3d microfluidic device for multiple bioassays. Paper substrates have been widely used to construct pointofcare lateral flow immunoassay diagnostic devices like the home pregnancy test. M bsa concentrations and 0250 mm glucose concentrations was used to investigate the relationship between two assays using the. Rise in demand for pointofcare poc devices is expected to significantly drive the market. Integrated paperbased microfluidic devices for pointof. This indicates that the smartphonebased 3d paperbased microfluidic device is a useful platform with great potential for application in pointofcare diagnostics. Paperbased systems for pointofcare biosensing sherine. Improving sample distribution homogeneity in three. Newgeneration hospital centers are equipped with centralized diagnostic laboratories containing hightech analytical devices and clinical instruments, which are not feasible or affordable for many lessdeveloped countries. Threedimensional microfluidic paperbased analytical devices 3d.
We will also identify challenges to commercialization. To listen to a podcast about this feature, please go to. Paper based microfluidic point of care diagnostic devices. Since the development of the first commercially available devices in the 1990s, microfluidic technologies have been evolving to benefit an evergreater range of applications. The integration issues relevant to the creation of paper based diagnostic devices are analogous to the issues in conventional microfluidics based diagnostic device development. Currently, the majority of poc diagnostics utilize paper substrates owing to affordability, disposability, and mass production capability. Pads are a new class of pointofcare diagnostic devices that are inexpensive, easy to use, and designed specifically for use in developing countries. From microfluidic paperbased analytical devices to paper. Microfluidics based devices need a fraction of the sample for data interpretation.